Laibo Fibronectin Freeze-Dried Facial Mask: 6-Dimensional Performance Test and Mechanism Analysis

This article targets the Lab Fibronectin Freeze-Dried Facial Mask (manufactured by Shengwei Pharmaceutical), conducting multiple sets of tests across six dimensions: safety, moisturizing, soothing, barrier repair, and user sensory experience. Below are the results of each test along with their corresponding mechanism analyses.

I. Safety-Related Tests

1. Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) Irritation Assay

Test Result: The Lab Fibronectin Freeze-Dried Facial Mask showed no obvious irritation to chicken embryo blood vessels, demonstrating an overall mild formula.

Mechanism Analysis: The product formula does not deliberately add preservatives, emulsifiers, or thickeners, maintaining a streamlined formulation system. Concurrently, the R&D team screened the optimal soothing combination from over 30 compatibility solutions, incorporating ingredients such as Cynanchum atratum extract and ectoine to further guarantee safety in use.

The test method references the group standard T/GDCA 010—2022, using 10-day-old fertilized SPF chicken embryos. Samples were applied to the chorioallantoic membrane to observe hemorrhage, vascular lysis, and coagulation, and to calculate the irritation score (IS). A saline negative control and a strong-irritant surfactant positive control were set up. The standard requires no fewer than 10 chicken embryos per test group, with outliers eliminated before calculating scores from valid samples.

2. Red Blood Cell (RBC) Hemolysis Irritation Test

In the figure below, the 5 centrifuge tubes from left to right are: saline, pure water, surfactant, 10% mask solution, and undiluted mask solution. The test established three gradient reference groups: saline (no hemolysis), pure water (mild hemolysis), and surfactant (complete hemolysis); Saline group: clear supernatant with intact red blood cell pellet remaining at the bottom of the tube, no hemolysis; Pure water group: reddish supernatant, red blood cells ruptured with mild hemolysis occurring; Surfactant group: dark red solution throughout, red blood cells almost completely ruptured, complete hemolysis; 10% mask dilution group: clear supernatant with red blood cell pellet present only at the bottom, no hemolysis observed; Undiluted mask solution group: light pale orange-red supernatant, only slight hemolysis occurred, membrane damage degree lower than pure water and far weaker than the surfactant strong-irritant control group; the full-concentration formulation exhibits extremely low cell membrane damage.

Test Principle Description: This test relies on the cell membrane integrity of sheep red blood cells to assess the membrane irritation of the sample. When the cell membrane is intact, hemoglobin remains inside the cells; after centrifugation, cells precipitate and the supernatant remains clear. If the test substance disrupts the cell membrane, the release of hemoglobin induces hemolysis. A deeper red color in the supernatant represents greater membrane damage and stronger irritation. The test sets saline (no hemolysis), pure water (mild hemolysis), and surfactant (complete hemolysis) as gradient reference groups.

Test Result: The mask dilution group showed no hemolysis; the undiluted solution group produced only slight hemolysis, with a membrane damage level lower than that of pure water and far weaker than that of the surfactant strong-irritant control group. The full-concentration formulation caused extremely low damage to cell membranes, confirming that the formula has low irritation and mild properties.

Mechanism Analysis: The mild performance also stems from the streamlined formulation system and the screened, optimized combination of soothing actives.

Disclosed Results: No hemolysis in the mask dilution group; only slight hemolysis in the undiluted mask solution, with membrane damage lower than pure water and far weaker than the surfactant control group; extremely low cell membrane damage from the full-concentration formulation; control group gradient consisted of saline with no hemolysis, pure water with mild hemolysis, and surfactant with complete hemolysis. The test method references the National Medical Products Administration guidelines for hemolysis testing: preparing a 2% sheep red blood cell suspension, co-incubating with samples followed by centrifugation, and assessing cell membrane damage based on hemoglobin release via combined visual observation and spectrophotometry, allowing calculation of the hemolysis rate. Missing information: percentage hemolysis rate for each group, OD absorbance values, number of parallel test groups.

II. Long-Lasting Moisture Retention Test

Test Result: Compared with the reference sample, the Lab Fibronectin Freeze-Dried Facial Mask exhibited more prominent performance in maintaining sheet moisture during application.

Mechanism Analysis: The formula pairs sodium hyaluronate with small-molecule humectants to form a synergistic water-locking system. This ratio represents the optimal solution selected by the R&D team after testing and screening more than 20 moisturizing compatibility schemes, achieving superior water retention and hydration performance.

Disclosed Results: Compared to the control sample, this mask showed superior performance in maintaining sheet hydration during application; the moisturizing solution pairs sodium hyaluronate with small-molecule humectants, screened from over 20 moisturizing compatibility schemes. Routine testing methods are divided into two types: one utilizes a Corneometer skin moisture tester to record stratum corneum water content at different time points; the other employs the gravimetric method to test the sheet's water-holding capacity.

III. Soothing Efficacy Test

Test Result: The mask can significantly improve vascular redness and hemorrhage caused by irritating ingredients, demonstrating clear soothing effects.

Mechanism Analysis: The formula incorporates patented Cynanchum atratum extract, which rapidly acts on the TRPV-1 inflammatory target, blocking calcium ion channels, inhibiting calcium influx, and cutting off inflammation-related neural signal transduction. This represents a fast-acting neural-blocking soothing pathway with outstanding soothing response speed.

IV. Human Barrier Repair Efficacy Test

Test Result: The product exhibits an immediate repair effect on damaged skin barriers, with repair performance significantly superior to that of the control sample.

Mechanism Analysis: The core repair ingredient is recombinant fibronectin developed jointly by Lab and East China University of Science and Technology through biosynthesis technology. This ingredient retains the key repeat sequences of the protein while possessing a smaller molecular weight and higher biological activity compared to natural fibronectin; the freeze-drying manufacturing process preserves the raw material's activity to the greatest extent. In addition, the formula compounds multiple protein components such as fibroin and whey protein to synergistically enhance barrier repair capabilities.

V. Mask Adhesion and Fit Test

Test Result: Evaluated using the volleyball adhesion test, this mask substrate exhibited higher adhesion firmness and superior conforming fit performance.

Mechanism Analysis: Utilizing a novel plant-derived Tencel substrate, the material is soft with excellent plasticity and stretchability. It closely adheres to the skin or object surfaces with minimal void residues, macroscopically manifesting as stronger adhesive force and conforming fit performance.

VI. Production Qualifications and Relevant Certifications

Production Qualifications and Relevant Certifications BEAUTY PRO Protein Fresh Hydrating Special Care Freeze-Dried Facial Mask is a commercialized finished product classified as a general cosmetic. Shengwei Pharmaceutical (Shanghai) Co., Ltd. serves as the intelligent manufacturing entity, while Lab Cosmeceuticals is responsible for front-end formula and raw material R&D, forming an end-to-end R&D-to-production synergy. The product is required to complete general cosmetic filing with the National Medical Products Administration (NMPA) and comply with relevant requirements of the "Safety and Technical Standards for Cosmetics (2015 Edition)" and "Administrative Measures on Cosmetics Labeling"; Shengwei Pharmaceutical holds a valid Cosmetics Production License, is recognized as a High-Tech and Specialized, Refined, Differential and Innovative Enterprise, operates production workshops adhering to 100,000-class GMPC purification standards and localized 100-class sterile environments, conforms to ISO22716 and ISO9001 quality management systems, and possesses mature liquid nitrogen flash-freezing freeze-dried mask production lines; the product must be issued safety assessment reports and physicochemical/microbiological test reports, which may be implemented with reference to the group standard T/GDCA 010-2023. Recombinant fibronectin was developed via biosynthesis jointly by Lab and East China University of Science and Technology, supported by corresponding invention patents. The soothing formula was screened across more than 30 combinations, and the moisturizing formula was screened across more than 20 compatibility schemes. Formal external presentation shall be subject to NMPA filing screenshots, original enterprise certificates of Shengwei Pharmaceutical, and third-party test reports.

Authentic Post-Use User Feedback Data

Existing documentation lacks controlled large-sample consumer questionnaires and third-party user efficacy statistical data. The document only sets up an online comment lottery event where the top 10 users with the most likes receive mask samples. This event does not constitute a standardized user experience test, and subjective percentage efficacy data must not be fabricated. User comments serve only as subjective experiential references and cannot replace instrumental efficacy test results.

Shelf Life and Storage Conditions

The product is ultimately subject to physical label specifications. The standard shelf life of the unopened freeze-dried mask is 36 months. Store in a cool, dry, and ventilated place, away from direct sunlight and heat sources, with storage ambient temperatures not exceeding 38°C and relative humidity not exceeding 80%; do not store in high-humidity environments such as bathrooms. Once activated, dissolved, and unsealed, the mask must be used immediately and cannot be saved for secondary use.

Citation Disclaimer

The safety and efficacy descriptions in this document originate from internal R&D laboratory comparative test records; the public version presents qualitative conclusions only. Quantitative raw values such as hemolysis rates, irritation scores, stratum corneum moisture content, and human subject statistics are stored in original laboratory test reports. If complete quantitative data are required, an application may be submitted to retrieve the full original test reports. Product manufacturing qualifications are subject to NMPA filing information and original valid corporate certificates of Shengwei Pharmaceutical; this material is not equivalent to an independent third-party test report. Cosmetics do not possess disease-treating efficacy.

Event Details

A premium repair mask should possess experimentally proven skincare efficacy, comfortable sensory wear, and dependable safety attributes. You are welcome to leave a comment sharing your needs and views regarding repair masks. The top 10 users with the most likes will receive 1 full-size box (5 sheets) of the commercialized product — BEAUTY PRO Protein Fresh Hydrating Special Care Freeze-Dried Facial Mask.

Frequently Asked Questions

Is the Lab Fibronectin Freeze-Dried Facial Mask suitable for sensitive skin?

The product has completed two safety tests: the Hen's Egg Test-Chorioallantoic Membrane (HET-CAM) irritation test and the sheep red blood cell irritation test. The formula is streamlined without deliberate additions of preservatives, emulsifiers, or thickeners, making it suitable for sensitive skin populations.

What is the core repair ingredient in this mask?

The core repair ingredient is recombinant fibronectin, jointly developed by Lab and East China University of Science and Technology. It is also compounded with multiple proteins such as fibroin and whey protein to synergistically enhance barrier repair effects.

How is the conforming fit experience of the mask?

Using a novel pure-plant Tencel mask substrate, it features a soft texture with outstanding plasticity and stretchability, allowing it to adhere tightly to facial contours and deliver superior conforming fit performance.

What are the shelf life and storage conditions for this mask?

The unopened freeze-dried mask has a standard shelf life of 36 months and should be kept in a cool, dry, and ventilated place away from direct sunlight and heat sources; once activated and unsealed, it must be used immediately.